Protein was extracted after induction, and samples were identified through western blot by using gene-specific as well as vector-specific antibodies
Protein was extracted after induction, and samples were identified through western blot by using gene-specific as well as vector-specific antibodies. amplified sites and vector were treated with restriction enzymes. For the construction of the NS3.1 expression vector, the gene and vector were treated with and restriction enzymes. In order to construct the NS5A.1 vector, the…