Residues corresponding to the hydrophobic spine M309, L320 and F401 are colored blue and rendered as sticks
Residues corresponding to the hydrophobic spine M309, L320 and F401 are colored blue and rendered as sticks. with trypsin (120, trypsin:protein) for 16 hours at 37C. The resulting peptides were analyzed with a Waters nanoAcquity UPLC system (1.0100.0 mm ACQUITY C18 BEH column) coupled to a Waters QTof Premier mass spectrometer. Peptide mass spectra were…