VH and V genes from each cell were amplified by RT-PCR and nested PCR reactions using cocktails of primers as previously described15,24 and then sequenced
VH and V genes from each cell were amplified by RT-PCR and nested PCR reactions using cocktails of primers as previously described15,24 and then sequenced. B cell receptor (BCR) repertoire that in some donors were dominated by only a few B cell clones. This pauci-clonal response, however, showed extensive intraclonal diversification from accumulated somatic mutations….