As negative and positive settings, PBMC were cultured in medium without peptide swimming pools or stimulated with PMA and calcium ionophore (Sigma, St
As negative and positive settings, PBMC were cultured in medium without peptide swimming pools or stimulated with PMA and calcium ionophore (Sigma, St. of commonly detected variants. In contrast to the full-length DNA vaccine, vaccination of macaques with a combination of these 2 Env CE DNA induced strong, durable cellular immune reactions with a significant…